DIGITALNA ARHIVA ŠUMARSKOG LISTA
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| ŠUMARSKI LIST 7-8/2023 str. 65 <-- 65 --> PDF |
Are oxidative stress screening tests enough for small scale Quercus robur genetic monitoring? Jesu li testovi praćenja oksidacijskog stresa dovoljni za genetički monitoring hrasta lužnjaka u malom razmjeru? Branislav Trudić, Biljana Kiprovski, Saša Kostić, Srđan Stojnić, Marko Kebert, Aleksandar Ivezić, Andrej Pilipović Summary Although being present much widely in terms of its geographical distribution, pedunculate oak (Quercus robur L.) represents one of the crucial species in forest ecosystems in South Eastern Europe. We wanted to contribute to existing efforts in designing genetic monitoring methodology for this noble broadleaved species, through the screening of genotypes with different health status and belonging to two phenological varieties [early (var. praecox) vs late (var. tardissima)] using seven oxidative stress parameters (total protein content, total phenolic compounds content, total flavonoids content, DPPH, ABTS, NO radical scavenger activities and Ferric reducing antioxidant power essay - FRAP). Results of this study provided significant biological message, contributing to better understanding of existing biodiversity of pedunculate oak in Serbia. Four out of seven oxidative stress screening tests (total phenolic compounds content, total flavonoids content, RSC NO and FRAP) clearly discriminated late vs early individuals sampled from selected pedunculate oak gene pool. Although presented results did not show significancy in using mentioned parameters to distinct damaged from vital individuals, nevertheless, knowledge gained through discussion and comparison with previous studies contributed to precise determination of biochemical tests which might be used in future efforts of genetic monitoring of this species. Key words: antioxidative, biodiversity, ecotype, pedunculate oak, stress INTRODUCTION UVOD Pedunculate oak (Quercus robur L.) is the dominant tree species in many natural and planted European lowland and riparian forests excluding large parts of Spain, Portugal and North Scandinavia (Sergeant et al., 2011). Among the deciduous, ring-porous oak species, pedunculate oak Q. robur is one of the most abundant species in Europe (Haneca et al., 2009). Q. robur is typically found in mesic sites across floodplains and valleys of large European rivers, lowlands and rocky sites (Madrigal-González et al., 2017). According to RCP climate scenarios, pedunculate oak (Q. robur L.) has been marked as the most affected tree species due to ongoing, intensive climate change (de Wergifosse, 2022). The decline of oak forests can cause great economical losses since oaks are considered highly valuable tree |