prilagođeno pretraživanje po punom tekstu

ŠUMARSKI LIST 11-12/2020 str. 43     <-- 43 -->        PDF

Physiological responses of two pedunculate oak (Quercus robur L.) families to combined stress conditions – drought and herbivore attack
Fiziološki odgovor dvije familije hrasta lužnjaka (Quercus robur L.) na kombinaciju stresa – suša i defolijatori
Andrej Pilipović, Milan Drekić, Srđan Stojnić, Nataša Nikolić, Branislav Trudić, Marina Milović, Leopold Poljaković-Pajnik, Milan Borišev, Saša Orlović
Pedunculate oak (Quercus robur L.) is economically and ecologically one of the most important tree species in lowland forests of Southeastern Europe, and it is endangered by numerous biotic and abiotic factors. In this study, we investigated the effect of drought and herbivore attack of gypsy moth (Lymantria dispar L.) on two families of young oak seedlings subjected to the following treatments: drought (D); gypsy moth (GM); both drought and gypsy moth (D+GM) and control (Ø) for a period of 15 days followed by a 7-day recovery period. During both treatment and recovery, physiological parameters - net photosynthesis (A), transpiration (E), stomatal conductance (gs), sub-stomatal CO2 concentration (Ci), water use efficiency (WUE), nitrate reductase activity (NRA) and chlorophyll content (Chl) were measured. Our results showed significant effects of stress factors on physiological processes in oak seedlings which could have potential impact on forest regeneration. Also, differences in the reaction between investigated families indicated the need for breeding and selection of more resistant progenies and provenances of pedunculate oak.
Key words: pedunculate oak, photosynthesis, WUE, SPAD, nitrate reductase activity
As one of a vast number of oak species, pedunculate oak (Quercus robur L.) is a species with a wide geographic distribution, starting from western Asia to Europe with reported scattered stands in Mediterranean regions (Gil Sanchez et al., 1994) that grow along the valleys of the major rivers in clear stands or mixed communities with narrow-leaved ash, hornbeam and elms. The pedunculate oak forests in regions of Slavonia and Srem in Western Balkans are well known all over the world for their valued quality wood for different industrial purposes. Nowadays climate change and regulation of watercourses significantly affect the

ŠUMARSKI LIST 11-12/2020 str. 44     <-- 44 -->        PDF

vitality of oak stands due to limitations in water availability (Stojanović et al., 2014; Stojanović et al., 2015; Kostić et al., 2019). Pedunculate oak is an especially interesting species for breeding and selection programs, taking into consideration its ecophysiology. Dependence of this species on soil water properties plays an uncertain role in its survival and stress response and, therefore, a focus on ecophysiology of this valuable broadleaved species should be a priority in research, breeding and forest management.
Survival and distribution of sessile organisms such as plants depend strongly on their ability to adjust to environmental variation (Beikircher and Mayr, 2009). Water stress, especially drought stress, is the main restriction of plant growth and development (Hu et al., 2004) where competition for water with mature trees may exacerbate drought effects on understory tree seedlings (Aranda et al., 2005). Water shortage and drought periods present limiting factors for forest regeneration in modern silvicultural practice by weakening the seedlings and affecting their performance at several levels. Besides direct effects on whole plant water status, drought causes reduction in stomatal aperture that diminishes CO2 supply to mesophyll cells, thus causing a reduction in photosynthetic rate (Blödner et al., 2007). However, stomatal limitation is not the only reason for decreased photosynthesis in drought-affected plants. According to Gallé et al. (2007) and the references within, the stomatal limitation predominately affects photosynthesis in plants under moderate drought conditions, whereas in severe drought metabolic limitations become dominant. Various parameters can be assessed in order to be more precise about whether the drought-induced photosynthetic decrease results from stomatal or metabolic limitations, such as chlorophyll a fluorescence (Gallé and Feller, 2007; Arend et al., 2013; Arend et al., 2016; Vastag et al., 2020), chlorophyll content (Gallé et al., 2007; Arend et al., 2012), biochemical markers (Gallé et al., 2007; Stojnić et al.,2019a) or even different non-structural carbohydrates and carbon isotope signatures (Pflug et al., 2018).
Among a vast number of factors, insects present an important biotic stressor that affects plant growth and vitality (Drekić et al., 2019). Outbreaks followed by and combined with climate extremes (e.g. temperature, precipitation, drought) will become more frequent due to the increase of greenhouse gas levels in the atmosphere. Plants, as sedentary organisms, cannot escape from attacks and stress and have to adjust to the surrounding environment and biotic attacks through their life cycle (Niinemets et al., 2013). Decreases in total leaf number and area are not the only response of plants to defoliation (Meyer, 1998; Poljaković-Pajnik et al., 2019), as grazing injury may also include a vast spectrum of metabolic and physical changes in host plants (Oleksyn et al., 1998), including enzymatic activities, gas exchange processes or accumulation of different metabolites like proline. Considering it as a significant determinant of plant growth, yield and fitness (Welter, 1989), plant gas exchange and influence of insects on it, present a significant aspect for further research in selection programs. On the other hand, close correlation of nitrogen metabolism with photosynthesis (Marschner, 1995) highlights nitrate reductase activity (NRA) as a good parameter for investigation since it is the first enzyme in nitrogen metabolism (Kastori and Petrović, 2003). Also, nutrition levels and the presence of allelochemicals in plants determine plant suitability and resistance to insect herbivores (Chen et al., 2011).
Gypsy moth (Lymantria dispar L.) is the most significant pest of hardwood forests in the northern hemisphere (Elkinton and Liebhold, 1990). The species naturally occurs in Eurasia but is also introduced in Northern America. Gypsy moth populations have occasional outbreaks lasting 4 to 5 years (Mihajlović, 2008) when caterpillars cause damage during leaf feeding on almost all forest tree species, except ashes. For its development, gypsy moth mostly prefers pure oak stands, with emphasis on Turkey oak (Q. cerris L.) (Milanović, 2006). Defoliation often causes losses in increment and acorn yield, as well as physiological weakening and susceptibility to secondary pests. In the case of consecutive defoliation, increases of increment loss and mortality occur (Mihajlović, 2008), where increment loss may range from 30-70%, according to Mirković and Miščević (1960).
Considering the importance of pedunculate oak for forest biodiversity and ecosystem restoration initiatives, constant efforts for its ex situ and in situ conservation through research of genetic diversity are of profound importance in order to mitigate climate change (Stojnić et al., 2019b). Apart from these efforts, breeding of more resilient provenances presents one of the efficient ways for future adaptation of forest tree species to climate change, since adaptation enables plants to optimize their life processes in prevailing environmental conditions at an evolutionary scale (Beikircher and Mayr, 2009). In order to achieve these long-term goals of pedunculate oak conservation and selection projects, integrating different research fields such as plant physiology, genetics and entomology presents a holistic, interdisciplinary solution that is appropriate for current and upcoming environmental challenges caused by climate change. Also, success in tree breeding can be facilitated by increased understanding of the physiology of growth and survival during water supply limitations (Wikberg and Ogren, 2007). Therefore, the goal of this research was to determine the simultaneous effect of drought stress and/or attack by caterpillars on the physiological traits of different families of pedunculate oak seedlings, during the stress period and after recovery.

ŠUMARSKI LIST 11-12/2020 str. 47     <-- 47 -->        PDF

Our results are in concordance with Aldea et al. (2006) who recorded a decline in photosynthesis of remaining leaf tissue in understory oak saplings, while Schaffer et al. (1997) recorded significant correlation between the damage intensity and net photosynthesis of citrus attacked by leaf miners. Besides a direct reduction of photosynthesis due to less leaf mass, defoliation indirectly reduces photosynthesis and transpiration of leaves by causing damage to vasculatures that supply leaf tissues (Nabity et al., 2009). Decrease of both photosynthesis and stomatal conductance in our study supported the above-mentioned hypothesis. On the other hand, Meyer and Whitlow (1992) found no alteration in photosynthesis and stomatal conductance in goldenrod under attack of leaf beetle, although significant increases in

ŠUMARSKI LIST 11-12/2020 str. 45     <-- 45 -->        PDF

Materials and Methods
Materijali i metode
Acorns of two families of pedunculate oak (Quercus robur L.) were collected from two single trees (Nos. 2 and 7) in a seed stand located at the territory of Public Enterprise Vojvodinašume; Forest Estate Sremska Mitrovica; Forest Management Unit Morović; during autumn 2017 and stored in a cooling chamber until March, when seeds were sown in 0.5-liter pots with soil medium consisting of a peat:sand:soil mixture of equal volumes (1:1:1). Prior to sowing, seeds were soaked in water for 24 hours in order to enhance germination. Plants were grown in the laboratory under constant light conditions until the beginning of May and then placed outside in insect cages where they were exposed to treatments. Before the start of the treatments, plants developed 5 to 10 leaves with height ranging from 15 to 35 cm.
The treatments included drought (D), damage caused by gypsy moth (Lymanthria dispar L.) (GM), and a combination of both stress factors (D+GM). Drought treatment (D) was preceded by a 15-day consecutive decrease of soil moisture to reach value of 30% of field capacity prior to the beginning of the treatment. Treatment with gypsy moth (GM) included 3 pieces of instar 2 caterpillars which were added to the plants in each pot. Both stress factors (D+GM) included addition of caterpillars to the drought stressed plants, while control treatment used well-watered plants without damages caused by the insects. Treatment lasted for 15 days followed by a 7-day recovery period. During recovery period, drought treated plants were irrigated to obtain optimal field capacity between 70 and 90%, while caterpillars were removed from the affected plants. Measurements of physiological factors were performed at the end of the 15-day treatment period and repeated after the 7-day recovery period. Measured parameters included gas exchange, pigments content and nitrate reductase activity (NRA). Each family had 10 pots per treatment from which four plants were selected for gas exchange measurements. Gas exchange measurements were made with a portable photosynthesis system (LCPro+, ADC Bioscientific, UK) under controlled constant light conditions of 1000 μmol m-2 s-1 and constant ambient air supply of 100 μmol s-1, with ambient levels of humidity and temperature. Measurements were performed on a fully developed leaf from the first set of leaves. For the gas exchanged measurement on insect damaged plants, selected leaves had a minimum of 50% damage with sufficient leaf blade area remaining for gas exchange measurements. Instantaneous water use efficiency (WUE [μmol mmol-1]) was computed as the ratio of net photosynthesis to transpiration (A/E) (Farquhar et al., 1989). Chlorophyll content was determined with the use of a portable chlorophyll meter (Minolta SPAD-502, Tokyo, Japan) on the same leaves where gas exchange measurements were performed. The SPAD values were converted to chlorophyll content (μg cm-2) according to Cerovic et al. (2012): Chl = (99 × SPAD value) (144 - SPAD value)-1. The in vivo NRA in leaves was assayed using the spectrophotometrical method of Hageman and Reed (1980) and expressed as µmol NO2- g-1FW h-1.
All statistical analyses were performed by STATISTICA software, version 13 (TIBCO Software Inc, 2017). Nested analysis of variance (ANOVA) was computed for each trait in order to evaluate relative importance of the following sources of phenotypic variation: date of measurement (the difference between performances of stressed and recovered seedlings), treatment nested within date (treatment effect during stress and recovery), family nested within date*treatment (genetic variation for seedlings response to stress and recovery). Significant differences were determined at p ≤ 0.05.
Response of physiological parameters to stress
Induced stress disturbed nitrogen metabolism in both investigated oak families. However, significant decrease of NRA was recorded in family 2, especially during drought treatment. During treatment, in family 2 values decreased by 55.1, 59.2 and 80.5% in plants exposed to GM, D+GM and D, respectively (Chart 1), compared to control. On the other hand, family 7 did not show significant decreases in NRA in all tested treatments although there were even increases in enzymatic activity in treatments D and GM. Net photosynthesis (A) decreased in both families (Chart 1). Significant decrease of A ranging from 42.9 to 61.2% compared to controls was recorded in both families in all treatments. Even though GM treatment had lowest values in both families, it did not significantly decrease compared to other stress treatments. Sub-stomatal concentration of CO2 (Ci) showed significant increases in GM- and D+GM-treated plants from family 2 (295.4 and 281.1 µmol µmol-1, respectively) and GM and D treated plants from family 7 (340.7 and 315.9 µmol µmol-1, respectively) (Chart 1). Gypsy moth attack decreased transpiration rate of both oak families during the treatment period (Chart 1), regardless if it was sole treatment (GM) or in combination with drought (D+GM). However, the decrease of stomatal conductance of family 2 D+GM plants was not significant (0.092 mol m-2 s-1). Within families, the change in WUE values (Chart 1) was not significant between the treatments, although decreases were recorded in plants of family 7 under separate effects of gypsy moth and drought (34.7% and

ŠUMARSKI LIST 11-12/2020 str. 46     <-- 46 -->        PDF

28.0%, respectively). Gypsy moth and water deficit differentially affected chlorophyll concentration in treated oak families (Chart 1). Values were lower in GM-treated family 2 plants versus the control. However, considerable changes in family 7 were evident only in plants under treatment D.
Recovery of physiological parameters after recovery period
Recovery period of all plants affected by stress factors (GM, D or D+GM) caused an increase in NRA of investigated families, both compared to the control and the measurements performed on stressed plants during the duration of the treatment (Chart 2). When compared with control, the increase was lowest for the GM treatment (49.3% for family 7 and 48.7% for family 2) and highest for the D+GM-treated plants (78.5% for family 7 and 73.3% for family 2) versus the control plants. Due to the increase of A in control plants in families 2 and 7 after recovery (from 6.59 to 9.24 µmolCO2 m-1 s-1 and from 6.44 to 7.35 µmolCO2 m-1 s-1, respectively), significant differences were recorded in treated plants after recovery period (Chart 2). After recovery Ci values were significantly higher in plants of family 7 under all stress treatments D, GM and D+GM (Chart 2), while Ci values in family 2 significantly decreased in drought recovered plants compared to control and treatments GM and D+GM. Stomatal conductance remained significantly decreased in D, GM and D+GM plants of family 2, while there were no significant differences between investigated plants of family 7. On the other hand, the transpiration rate remained decreased in all treatments after the recovery period. After recovery, plants from family 7 did not have significantly-decreased WUE, although values for GM, D and D+GM were lower than in control plants (3.13, 2.91, 3.63 and 4.21 µmolCO2 µmolH2O-1, respectively). Affected plants from family 2 had significantly smaller values of WUE in GM treatment (1.65 µmolCO2 µmolH2O-1) when compared to control plants from the same family (Chart 2). Watering of treated plants substantially affected chlorophyll content of the oak family 2 (Chart 2). Recovered plants under D and GM treatment had significantly lower chlorophyll content when compared to control (26.8, 25.0 and 34.5 µg cm-2, respectively). Chlorophyll content of recovered plants of family 7 did not differ significantly from the control plants.
Influence of the family, treatment, recovery and their interaction on the investigated physiological parameters
In most of the investigated parameters, origin of the seed (family) showed significant influence on the results. Nested ANOVA results (Table 1) showed significant impact of all calculated factors for NRA: origin (F=7.76***), treatment (F=26.31***) and recovery (F=665.0***). Analysis of variance showed that the effect of treatment was only significant for A (F=38.50*** at p<0.001), while the recovery period (date) and families showed no significant effect on net photosynthesis (F=3.51ns and F=1.17ns, respectively). On the other hand, sub stomatal concentration of CO2 (Ci) followed a similar pattern as A only for recovery time which had no effect on Ci (F=0.57ns), but origin of seedling significantly affected Ci (F=28.24*** at p<0.001). Transpiration rate (E) and stomatal conductance (gs) were both affected by the treatment and dependent upon the origin of plants (Table 1), while recovery period did not show an effect on gs (F=0.06ns). Analysis of variance of WUE of treated plants (Table 1) showed significant impact of all three factors: recovery time (F=16.21***), treatment (F=6.30***) and origin of plants (F=8.22***). Results of nested ANOVA (Table 1) showed significant effects, both of treatment (F=11.44***) and family (F=10.04***), while recovery period did not significantly affect chlorophyll content of the trees.
Vegetation responses to environmental conditions are mediated by a suite of functional traits affecting water relationships, resource acquisition and other aspects of plant function (Manzoni, 2014). Increase in photosynthetic rate commonly occurs following defoliation events, which is considered a photosynthetic up-regulation caused by the disturbance of a source:sink ratio and increased demand for carbohydrates to rebuild crowns (Pinkard et al., 2007). Various authors found opposite plant photosynthetic responses during defoliation. Peterson et al. (1996) found no alteration of photosynthesis in hardwoods under pest attack, while Turnbull et al. (2007) recorded increased photosynthesis in leaves of eucalyptus (Eucalyptus globulus Labill.) under partial defoliation. Decreased photosynthesis was recorded under defoliation treatments in this study.

ŠUMARSKI LIST 11-12/2020 str. 48     <-- 48 -->        PDF

sub-stomatal CO2 concentration suggested a possible decrease of photosynthetic rate. The decrease in photosynthesis and stomatal conductance in our study was also followed by decreases of Ci under defoliation. Following analogy of the abiotic stress impact (i.e. drought) on the limitation of photosynthesis recorded in various papers (Gallé et al., 2007; Gallé and Feller, 2007; Haldimann et al., 2008; Arend et al., 2013; Arend et al., 2016), increases in Ci could be defined as a metabolic limitation of photosynthetic process of gypsy moth attacked leaves.
The decrease in photosynthesis of young oak seedlings under drought treatment is difficult to interpret and generalize because, besides the factor of treatment and date, the origin of seedlings plays a significant role given genetic effect on the physiological performance of the plants. Correlation between the genetic background and leaf gas exchange parameters is very well documented (Orlović et al., 1998), which often causes different physiological responses of the individuals within the same species to unfavorable conditions (Pilipović et al., 2014; Pointeau and Guy, 2014; Bojović et al., 2017; Vastag et al., 2019). Alterations of photosynthetic parameters differed between the families, where decreases in A in family 2 were not followed by increases in Ci like in family 7. Such results indicate possible higher drought sensitivity of family 7, compared to family 2. Similar results were observed by Haldimann et al. (2008) in Quercus pubescens L. who hypothesized that drought-induced metabolic limitations and drought-dependent increases in mesophyll resistance to the diffusion of CO2 were present. As mentioned previously, sub-stomatal concentrations of carbon dioxide (Ci) together with stomatal conductance (gs) indicate mechanisms and severity of photosynthetic inhibition where decreased Ci is related with stomata and increased Ci is described as a metabolic limitation of photosynthesis. Different studies (Lawlor, 1995; Gallé et al., 2007; Mathobo et al., 2017) confirmed that an intense drought leads to an increase in the values of Ci, while the conditions of a moderate drought result in reduction of Ci. Considering the results of this study, drought treatment affected Ci values in different manners, emphasizing the importance of genetic background in understanding drought responses of tested families. Opposite to photosynthesis, drought did not affect transpiration of investigated oak families. Bréda et al. (1993) concluded that oaks were drought-tolerant species due to their ability to maintain significant transpiration intensity under reduced water availability in the soil. This corroborates results obtained in this study (i.e., transpiration did not decrease under drought treatment).
As expected, the presence of stress factors affected metabolic processes in both investigated oak families. On the other hand, expected synergistic effects of both drought and defoliation were not observed in this study, probably due to the various expressions of simultaneously occurring stressors. According to Copolovici et al. (2014), one type of stress could weaken or enhance the effects of another simultaneous stress factor by direct physiological cumulative or interactive effects. In contrast, La Spina et al. (2010) emphasized a lack of expected parabolic response of herbivore performance to tree water status.
Despite chlorophyll a and b being highly sensitive to decreased soil moisture (Farooq et al., 2009) and that drought-induced reductions in pigment contents were previously found in many woody plant species (Lei et al., 2006; Gallé and Feller, 2007; Guerfel et al., 2009, Arend et al., 2013), chlorophyll contents of tested families did not respond similarly. In family 7 D treatment decreased chlorophyll content, while a significant decrease of pigments in family 2 was recorded only under gypsy moth defoliation (GM). Similar observation also was noted during summer drought in Q. robur and Q. petraea (Epron and Dreyer, 1993). According to Rahdari and Hoseini (2012), an increase in chlorophyll levels under conditions of environmental stress is one of the resistant symbols in plants that are proportional to stress. Since family 2 had a higher content of pigments under drought treatment than family 7, the results indicated that those plants may have had a higher drought tolerance and provided a stronger photoprotective system against drought stress than plants from family 7.
It is very well documented that NRA generally decreases in leaves of plants subjected to water stress (Kaiser and Förster, 1989; Foyer et al., 1998; Garg et al., 2001). During water deficit, NRA decreases more rapidly than most enzymes (Huffaker et al.,1970) and often presents more sensitive physiological indicators of water stress than either stomatal closure or photosynthesis (Bardzik et al., 1971; Hsiao et al., 1976). The negative effect of water deficit on the activity of this enzyme may result from decreased nitrate reductase protein or decreased activation of the existing protein (Correia et al., 2005). In our experiment, compared to the control, NRA appears to vary significantly in the leaves of family 2 in response to drought treatment. On the other hand, family 7 did not express decrease in any of the treatments. One of the reasons for this may be the fact that compatible solutes may contribute to the maintenance of enzyme activity. According to Smirnoff et al. (1985), accumulation of proline may facilitate the continued synthesis of nitrogenous compatible solutes using excess photochemical energy available when stomata are closed, as recorded in family 7. In addition to carbon metabolism, nitrogen metabolism is also affected by drought stress.
Recovery period still showed a significant decrease of net photosynthesis and transpiration in both investigated

ŠUMARSKI LIST 11-12/2020 str. 49     <-- 49 -->        PDF

families. However, differences between stomatal conductance and intercellular CO2 concentrations among families were evident, indicating genetic background of their recovery. Compared to control, recovered plants of family 2 showed no increase of Ci, while stomatal conductance remained low, indicating cessation of metabolic limitation of photosynthesis. Decreases of stomatal conductance recorded in family 2 can be attributed to the inability of stomata to completely re-open (Kozlowski, 1982) or to the structural changes in stomata (Gallé and Feller, 2007). The increase of NRA after the 7-day period indicated an ongoing recovery process in the stressed plants, which is consistent with previous studies (Bardzik et al., 1971; Ferrario-Méry et al., 1998; Foyer et al., 1998; Correia et al., 2005).

ŠUMARSKI LIST 11-12/2020 str. 50     <-- 50 -->        PDF

Although at first glance various research (Gallé et al., 2007; Gallé and Feller, 2007; Haldimann et al., 2008; Arend et al., 2013; Arend et al., 2016) indicated slightly opposite results on the recovery of the forest tree species after stress, deeper analysis of the both obtained and quoted results show similarities and concordance. Arend et al. (2013) showed that the drought affected the physiological processes and their recovery in Q. robur to a greater extent than in Q.petreae and Q. pubescens. Net photosynthesis and chlorophyll content were strongly affected by drought, and the recovery period was prolonged. Comparing our results with aforementioned research, it can be suggested that the 7-day recovery period was not sufficient for oaks to restore their physiological processes to optimum. In most cases, recovery period can vary upon the origin of the seedlings where provenances from xeric sites had shorter recovery period (Arend et al., 2016), or in some cases, atmospheric conditions such as elevated CO2 can accelerate recovery of the photosynthesis (Gallé et al., 2007). Sometimes even when the net photosynthesis recovers fully, the recovery of the entire photosynthetic process can be prolonged due to the disturbance in stomatal performance (Gallé and Feller, 2007).
Obtained results showed significant decrease of net photosynthesis of investigated oak families under the simultaneous effect of drought stress and/or attack by caterpillars. However, NRA did not decrease under stress in both families. Recovery period did not increase net photosynthesis in plants but showed differences in limitation (stomatal/metabolic) between families. On the other hand, nitrate reductase activity was increased in stressed plants of both oak families indicating beginning of recovery. Based on comparison of the recovery measurements data and existing literature it can be concluded that the 7-day recovery was insufficient for oak seedlings to fully restore their optimal physiological processes. Analysis of data also showed that there is a significant genetic effect in relation to the families’ response to induced stress and recovery, indicating the possibility to use physiological parameters in the selection of stress-tolerant oak progenies and provenances.
This study was financed by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project No: 451-03-68/2020-14/ 200197). We would like to thank Mr Ed Bauer and Dr Ron Zalesny from the USDA Forest Service, Northern Research Station, Institute for Applied Ecosystem Studies, Rhinelander, WI USA for English and style editing.
Aldea, M., J.G. Hamilton, J.P. Resti, A.R. Zangerl, M.R. Berenbaum, T.D. Frank, E.H. DeLucia, 2006: Comparison of photosynthetic damage from arthropod herbivory and pathogen infection in understory hardwood saplings, Oecologia, 149: 221–232.
Aranda, I., L. Castro, M. Pardos, L. Gil, J.A. Pardos, 2005: Effects of the interaction between drought and shade on water relations, gas exchange and morphological traits in cork oak (Quercus suber L.) seedlings, Forest Ecology and Management, 210(1–3): 117-129.
Arend, M., A. Brem, T.M. Kuster, M.S. Günthardt‐Goerg, 2013: Seasonal photosynthetic responses of European oaks to drought and elevated daytime temperature. Plant Biology, 15: 169-176. doi:10.1111/j.1438-8677.2012.00625.x
Arend, M., K. Sever, E. Pflug, A. Gessler, M. Schaub, 2016: Seasonal photosynthetic response of European beech to severe summer drought: Limitation, recovery and post-drought stimulation. Agricultural and Forest Meteorology, 220: 83-89.
Beikircher, B., S. Mayr, 2009: Intraspecific differences in drought tolerance and acclimation in hydraulics of Ligustrum vulgare and Viburnum lantana, Tree Physiology, 29(6): 765–775.
Bardzik, J.M., H.V.J. Marsh, J.R. Havis, 1971: Effects of water stress on the activities of three enzymes in maize seedlings, Plant Physiology, 47: 828-831.
Blödner, C., A. Majcherczyk, U. Kües, A. Polle, 2007: Early drought-induced changes to the needle proteome of Norway spruce, Tree Physiology 27: 1423–1431.
Bojović, M., N. Nikolić, M. Borišev, S. Pajević, M. Župunski, R. Horak, A. Pilipović, S. Orlović, S. Stojnić, 2017: The diurnal time course of leaf gas exchange parameters of penduculate oak seedlings subjected to experimental drought conditions, Baltic Forestry, 23(3): 584-594.
Bréda, N., H. Cochard, E. Dreyer, A. Granier, 1993: Seasonal evolution of water transfer in a ma-ture oak stand (Quercus peiraea Matt. Liehl.) submitted to drought, Canadian Journal of Forest Research, 23: 1136-1143.
Cerovic, Z.G., G. Masdoumier, N. Ben Ghozlen, G. Latouche, 2012: A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids, Physiologia Plantarum, 146(3): 251-260.
Chen, Z.Y., Z.S. Peng, J. Yang, W.Y. Chen, Z.M. Ou-Yang, 2011: A mathematical model for describing light-response curves in Nicotiana tabacum L., Photosynthetica, 49 (3): 467-471.
Copolovici, L., F. Väärtnõu, M. Portillo-Estrada, U. Niinemets, 2014: Oak powdery mildew (Erysiphe alphitoides)-induced volatile emissions scale with the degree of infection in Quercus robur, Tree Physiology, 34(12): 1399-1410.
Correia, M.J., F. Fonseca, J. Azedo-Silva, C. Dias, M.M. David, I. Barrote, M.L. Osório, J. Osório, 2005: Effects of water deficit on the activity of nitrate reductase and content of sugars, nitrate and free amino acids in the leaves and roots of sunflower and white lupin plants growing under two nutrient supply regimes, Physiologia Plantarum, 124: 61-70.
Drekić, M., L. Poljaković–Pajnik, V. Vasić, B. Kovačević, M. Marković, M. Milović, A. Pilipović, 2019: Short-term prognosis of ash weevil damages, Topola/Poplar 2019, 204: 51-57.

ŠUMARSKI LIST 11-12/2020 str. 51     <-- 51 -->        PDF

Efeoğlu, B., Y. Ekmekçi, N. Çiçek, 2009: Physiological responses of three maize cultivars to drought stress and recovery, South African Journal of Botany, 75: 34-42.
Elkinton, J. S., A.M. Liebhold, 1990: Population dynamics of gypsy moth in North America, Annu. Rev. Entomol., 35: 571-596.
Epron, D., E. Dreyer, 1993: Compared effects of drought on photosynthesis of adult oak trees (Quercus petraea (Matt) Liebl, Q rubra L. and Q cerris L.), New Phytologist, 125: 381-389.
Farooq, M., A. Wahid, N. Kobayashi, D. Fujita, S.M.A. Basra, 2009: Plant drought stress: effects, mechanisms and management, Agronomy for Sustainable Development, 29: 185-212.
Farquhar, G.D., J.R. Ehleringer, K.T. Hubick, 1989: Carbon isotope discrimination and photosynthesis, Annual Review of Plant Physiology and Plant Molecular Biology, 40: 503–537.
Ferrario-Méry, S., M.H. Valadier, C.H. Foyer, 1998: Overexpression of nitrate reductase in tobacco delays drought-induced decreases in nitrate reductase activity and mRNA, Plant Physiology, 117: 293-302.
Foyer, C.H., M.-H. Valadier, A. Migge, T.W. Becker, 1998: Drought induced effects on nitrate reductase activity and mRNA on the coordination of nitrogen and carbon metabolism in maize leaves, Plant Physiology, 117: 283-292.
Gallé, A., P. Haldimann, U. Feller, 2007: Photosynthetic performance and water relations in young pubescent oak (Quercus pubescens) trees during drought stress and recovery. New Phytologist, 174: 799-810. doi:10.1111/j.1469-8137.2007.02047.x
Gallé, A., U. Feller, 2007: Changes of photosynthetic traits in beech saplings (Fagus sylvatica) under severe drought stress and during recovery. Physiologia Plantarum, 131: 412-421. doi:10.1111/j.1399-3054.2007.00972.x
Garg, B.K., S. Kathju, U. Burman, 2001: Influence of water stress on water relation, photosynthetic parameters and nitrogen metabolism of moth bean genotype, Biologia Plantarum, 44: 289-292.
Gil Sánchez, L., M.P. Jiménez Sancho, P.M. Diaz-Fernandez, 1994: Quercus complex in Spain: an overview of its present state. Inter-and intra-specific variation in European oaks: evolutionary implications and practical consequences, Brussels.
Guerfel, M., O. Baccouri, D. Boujnah, W. Chaibi, M. Zarrouk, 2009: Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars, Scientia Horticulturae, 119: 257-263.
Hageman, R.H., A.J. Reed, 1980: Nitrate reductase for from higher plants, Methods in Enzymology, 69: 270-279.
Haldimann, P., A. Gallé, U. Feller, 2008: Impact of an exceptionally hot dry summer on photosynthetic traits in oak (Quercus pubescens) leaves, Tree Physiology, 28(5): 785–795.
Hsiao, T.C., E. Acevedo, E. Fereres, D.W. Henderson, 1976: Water stress, growth and osmotic adjustment, Philosophical Transactions of the Royal Society B, 237: 479-500.
Hu, J.C., W.X. Cao, J.B. Zhang, 2004: Quantifying responses of winter wheat physiological processes to soil water stress for use in growth simulation modeling, Pedosphere, 14: 509–518.
Huffaker, R.C., T. Radin, G.E. Kleinkopf, E.L. Cox, 1970: Effects of mild water stress on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley, Crop Science, 10: 471-474.
Kaiser, W.M., J. Förster, 1989: Low CO2 prevents nitrate reduction in leaves, Plant Physiology, 91: 970–974.
Kastori, R., N. Petrović, 2003: Nitrati u povrću: fiziološki, ekološki i agrotehnički aspekti; Naučni institut za ratarstvo i povrtarstvo - Novi Sad, Verzal, Novi Sad, Jugoslavija
Kostić, S., T. Levanič, S. Orlović, B. Matović, D.B. Stojanović, 2019: Pedunculate and turkey oaks radial increment and stable carbon isotope response to climate conditions through time, Topola/Poplar, 204: 29-35.
Kozlowski, T.T. 1982: Water supply and tree growth. Part I: Water deficits, Forestry Abstracts, 43: 57-95.
La Spina, S., J. Gregoire, P. Mertens, C. De-Canniere, 2010: Impact of poplar water status on leaf-beetle (Chrysomela populi) survival and feeding, Ann. For. Sci., 67,209.
Lawlor, D.W., 1995: The effects of water deficit on photosynthesis. In: Smirnoff N. (ed) Environment and Plant Metabolism. Flexibility and Acclimation, Bios ScientificPub-lishers, pp. 129–160., Oxford.
Lei, Y., C. Yin, C. Li, 2006: Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii, Physiologia Plantarum, 127: 182-191.
Manzoni, S., 2014: Integrating plant hydraulics and gas exchange along the drought-response trait spectrum, Tree Physiology, 34(10): 1031–1034,
Marschner, H., 1995: Mineral Nutrition of Higher Plants, 2nd edn. Academic Press, London, UK.
Mathobo, R., D. Maraisa, J.M. Steyn, 2017: The effect of drought stress on yield, leaf gaseous ex-change and chlorophyll fluorescence of dry beans (Phaseolus vulgaris L.), Agricultural Water Management, 180: 118-125.
Meyer, G.A., T.H. Whitlow, 1992: Effects of leaf and sap feeding insects on photosynthetic rates of goldenrod, Oecologia, 92: 480-489.
Mihajlović, LJ., 2008: Gubar (Lymantria dispar L.) (Lepidoptera: Lymantridae) u Srbiji, Šumarstvo, 60(1-2): 1-26. (Serbian with English summary)
Milanović, S., 2006: Uticaj vrsta Quercus cerris L., Q. petrea Liebi. i Q. robur L. na razviće gubara (Lymantria dispar L.) (Masters’s thesis), Faculty of Forestry, Belgrade. (Serbian with English summary) Beograd.
Mirković, D., V. Miščević, 1960: Uticaj brsta gubara (Lymantria dispar L.) na prirast hrasta, Zaštita bilja, 60: 3-19. (Serbian with English summary).
Nabity, P. D., J. A. Zavala, E. H. DeLucia, 2009: Indirect suppression of photosynthesis on individual leaves by arthropod herbivory, Annals of Botany, 103(4): 655–663.
Niinemets, Ü., A. Kännaste, L. Copolovici, 2013: Quantitative patterns between plant volatile emissions induced by biotic stresses and the degree of damage, Frontiers in Plant Science, 4: 262
Oleksyn, J., P. Karolevski, M.J. Giertych, R. Zytowiak, P.B. Reich, M.G. Tjoelker, 1998: Primary and secondary host plants differ in leaf-level photosynthetic response to herbivory: evidence from Alnus and Betula grazed by the alder beetle, Agelastica alni, New Phytologist, 140: 239-249.
Orlović, S., V. Guzina, B. Krstic, Lj. Merkulov, 1998: Genetic variability in anatomical, physiological and growth characteristics

ŠUMARSKI LIST 11-12/2020 str. 52     <-- 52 -->        PDF

of hybrid poplar (Populus × euramericana DODE (GUINIER)) and eastern cottonwood (Populus deltoides BARTR.) clones, Silvae Genetica, 47: 183-190.
Panda, A.K., V.S. Dagaonkar, M.S. Phalak, G.V. Umalkar, L.P. Aurangabadkar, 2007: Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery, Plant Biotechnology Reports, 1: 37-48.
Peterson, R.K.D., L.G and Higley, 1996: Temporal changes in soybean gas exchange following simulated insect defoliation, Agronomy Journal, 88: 550-554.         
Pflug, E., N. Buchmann, R. Siegwolf, M. Schaub, A. Rigling, M. Arend, 2018: Resilient Leaf Physiological Response of European Beech (Fagus sylvatica L.) to Summer Drought and Drought Release. Frontiers in Plant Science, 9: 187.
Pilipović, A., M. Drekić, S. Orlović, L. Poljaković-Pajnik, N. Nikolić, M. Borišev, 2014: Growth and Physiological Response of Different Poplar Clones on Herbivory Induced Stress. Proceedings of the Biennial International Symposium “Forest and sustainable development”, Brașov, Romania: 121-126.
Pinkard, E. A., M. Battaglia, C.L. Mohammed, 2007: Defoliation and nitrogen effects on photosynthesis and growth of Eucalyptus globulus, Tree Physiology, 27(7): 1053–1063.
Pointeau, V.M., R.D. Guy, 2014: Comparative resource-use efficiencies and growth of Populus trichocarpa and Populus balsamifera under glasshouse conditions, Botany, 92:443–451.
Poljaković-Pajnik, L., M. Drekić, B. Kovačević, M. Stanković- Neđić, S. Stojnić, S. Orlović, 2019: Host preference of Myzus cerasi (Fabricius, 1775) to half-sib lines of Prunus avium L. from six populations assessed in the nursery trial, Topola/Poplar, 203: 87-94.
Rahdari, P., S.M. Hoseini, 2012: Drought stress: A review, International Journal of Agronomy and Plant Production, 3(10): 443-446.
Schaffer, B., J. E. Penã, A. M. Colls, A. Hunsberger, 1997: Citrus leafminer (Lepidoptera: Gracillariidae) in lime: assessment of leaf damage and effects on photosynthesis, Crop. Prot., 16: 337–343.
Smirnoff, N., M.D. Winslow, G.R. Stewart, 1985: Nitrate reductase activity in leaves of barley (Hordeum vulgare) and durum wheat (Tricitum durum) during field and rapidly applied water deficits, Journal of Experimental Botany, 36: 1200-1208.
Stojanović, D., B. Matović, S. Orlović, S., A. Kržić, B. Trudić, Z. Galić, S. Stojnić, S. Pekeč, 2014: Future of the main important forest tree species in Serbia from the climate change perspective, South-east European Forestry 5(2): 117-124.
Stojanović, D., T. Levanič, B. Matović, S. Orlović, 2015: Growth decrease and mortality of oak floodplain forests as a response to change of water regime and climate, European Journal of Forest Research, 134(3): 555-567.
Stojnić, S., B. Kovačević, M. Kebert, E. Vaštag, M. Bojović, M.S. Neđić, S. Orlovic, 2019a: The use of physiological, biochemical and morpho-anatomical traits in tree breeding for improved water-use efficiency of Quercus robur L., Forest Systems, 28(3): e017.
Stojnić, S., S. Orlović, A. Pilipović, 2019b: Ex situ conservation of forest genetic resources in Serbia. In: Šijačić-Nikolić, M., Milovanović, J., Nonić, M. (Eds.). Forests of Southeast Europe under a changing climate. Conservation of forest genetic resources. Springer Nature Switzerland AG, pp. 227-237.
TIBCO Software Inc, 2017: Statistica (data analysis software system), version 13. URL:
Turnbull, T. L., M. A. Adams, C. R. Warren, 2007: Increased photosynthesis following partial defoliation of field-grown Eucalyptus globulus seedlings is not caused by increased leaf nitrogen, Tree Physiology, 27: 1481-1492.
Vastag, E., L. Kesić, S. Orlović, V. Karaklić, M. Zorić, V. Vuksanović, S. Stojnić, 2019: Physiological performance of sweetgum (Liquidambar stryraciflua L.) and norway maple (Acer platanoides L.) under drought condition in urban environment, Topola/Poplar, 204: 17-27.
Vastag, E., C. Cocozza, S. Orlović, L. Kesić, M. Kresoja, S. Stojnić, 2020: Half-sib lines of pedunculate oak (Quercus robur L.) respond differently to drought through biometrical, anatomical and physiological traits. Forests, 11(2): 153.
Welter, S.C., 1989: Arthropod impact on plant gas exchange. In: Insect–Plant Interactions (eds.Bernays, E.A.) Vol. 1., CRC Press, 135–150., Boca Raton, USA.
Wikberg, J., E. Ogren, 2007: Variation in drought resistance, drought acclimation and water conservation in four willow cultivars used for biomass production, Tree Physiology, 27: 1339-1346.
Hrast lužnjak (Quercus robur L.) predstavlja jednu od ekonomski i ekološki najznačajnijih drvenastih vrsta u jugoistočnoj Europi, čiji je opstanak znakovito ugrožen promjenama okoliša koje se najčešće očituju u poremećaju vodnog režima i napadima kukaca. Imajući u vidu činjenicu da oba negativna čimbenika dovode do poremećaja fizioloških procesa u biljkama i da često utječu na biljke zajedno, cilj ovoga pokusa bio je ispitati utjecaj pojedinačnog i zajedničkog djelovanja suše i napada kukaca na fiziologiju hrasta lužnjaka i mogućnost različite reakcije uvjetovane porijeklom sadnica.
U ovom smo radu ispitivali utjecaj suše i napada gubara (Lymantria dispar) na klijance hrasta lužnjaka porijeklom iz dvije familije polusrodnika koji su bili naklijani u kontroliranim uvjetima i potom izloženi sljedećim tretmanima: suša ( D); gubar (GM); suša i gubar (D+GM) te kontrola (Ø) u trajanju od 15 dana, nakon čega je slijedio oporavak biljaka u trajanju od 7 dana. Krajem tretmana i nakon oporavka, izvršena su mjerenja fizioloških parametara – stopa neto fotosinteze (A), stopa transpiracije (E), stopa provodljivosti puči (gs), intercelularna koncentracija CO2 (Ci), učinkovitosti korištenja vode (WUE), aktivnosti nitrat-reduktaze (NRA) i sadržaja klorofila.

ŠUMARSKI LIST 11-12/2020 str. 53     <-- 53 -->        PDF

Rezultati mjerenja fizioloških parametara prilikom induciranog Kod tretiranih klijanaca A opala je za 42-61% u odnosu na kontrolni tretman (Graf 1) a u isto vrijeme, zabilježen je porast Ci u svim tretmanima, što ukazuje na metaboličku limitiranost fotosinteze, osobito ako se uzme u obzir da nije zabilježeno signifikantno smanjenje provodljivosti puči u svim tretmanima. Inducirani stres je različito utjecao na aktivnost nitrat-reduktaze koja je bila smanjena samo kod familije 2 u svim tretmanima. Period oporavka biljaka (Graf 2) nije doveo do povećanja intenziteta fotosinteze kod ispitivanih sijanaca hrastova u obje familije, ali je zabilježena razlika u pogledu razine njene limitiranosti. Kod familije 2 limitiranost je bila uvjetovana zatvorenošću puči (smanjena vrijednost gs), dok je kod familije 7 ona i dalje bila metaboličke prirode (visoke vrijednosti Ci). Oporavak je uvjetovao povećanje razine aktivnosti nitrat-reduktaze, što ukazuje na postepeni oporavak biljaka.
Analiza varijance utjecaja tretmana, familije i perioda oporavka (Tablica 1) pokazala je znakoviti utjecaj porijekla sjemena na sve ispitivane parametre osim intenziteta neto fotosinteze (p>0.001), dok je utjecaj tretmana bio signifikantan kod svih ispitivanih parametara (p>0.001) Period oporavka je imao signifikantan utjecaj (p<0.001) samo za E, NRA i WUE.
Dobiveni su rezultati pokazali značajan utjecaj stresa na fiziološke procese u klijancima hrasta lužnjaka, posebice na intenzitet neto fotosinteze, dok je oporavak pokazao različitu reakciju ispitivanih familija unatoč i dalje smanjenom intenzitetu neto fotosinteze. Dobiveni rezultati ukazuju i na značaj porijekla sjemena, odnosno utjecaj njegove genetske pozadine na reakciju ispitivanih familija glede njihove reakcije i oporavka od stresa. To ukazuje na mogućnost korištenja fizioloških parametara u procesu selekcije reproduktivnog materijala hrasta lužnjaka na otpornost prema stresnim čimbenicima poput suše ili napada defolijatora.
Ključne riječi: hrast lužnjak, fotosinteza, WUE, SPAD, aktivnost nitrat-reduktaze