DIGITALNA ARHIVA ŠUMARSKOG LISTA
prilagođeno pretraživanje po punom tekstu
| ŠUMARSKI LIST 9-10/2020 str. 33 <-- 33 --> PDF |
Laboratory and Greenhouse experiments – Tretmani u laboratoriju i u stakleniku In this study, treatments of growth chamber and greenhouse are shown in Table 1. Laboratory Experiments – Istraživanje u laboratoriju The seeds were soaked for 48 hour in water at room temperature (22°C ± 2°C) (Genc 2012) before germination and sowing treatments in order to break dormancy caused by seed coat. Also, the seeds were treated with cold-moist stratification treatment to break seed dormancy. The seeds were mixed with approximately % 40 humidified sand and placed in plastic bags, and then stored in the refrigerator (at 40C) for cold-moist stratification treatment (Saatcioglu 1971; Yahyaoglu and Olmez 2005). In pre-experiments, the highest germination percentage of stratified seeds was obtained in seeds treated with stratification for 8 weeks. Therefore, stratification period was determined as 8 weeks in this study. As a different treatment, the seeds collected different collection time were treated with GA3 (Giberellic acid; 50,100,400 ppm) for 24 hours and germinated in growth chamber (Table 1). Germination tests were conducted in petri dishes (ISTA 1996) and 100 (4 X 25) seeds were used for each germination test. Petri dishes and filter paper were sterilized in the oven at 160 oC for approximately 2 hours. Petri dishes covered and randomly placed in growth chamber. The seeds with radicles longer than 3 mm were tought to be germinated and taken from the petri dishes (Jensen 2001). Germination tests were considered completed when there was no germination for 14 consecutive days (Tremblay et al. 1996). In pre-experiments, the highest germination percentage of Acer trautvetteri seeds was observed in germination experiments at + 5 0C. Therefore, in this study, the germination experiments were carried out at + 5 0C. Greenhouse Experiments – Istraživanja u stakleniku In order to evaluate the germination performance of Acer trautvetteri seeds in the greenhouse conditions, seed beds (soil ) were used in the greenhouse at East Black Sea Forestry Research Institue. The seeds were subjected to soaking 48 hours water, 8 week stratification, soaking 48 hours water + 8 week stratification and GA3 applications (100 and 400 ppm) (Table 1).100 seeds were used for each treatment. The seeds were sown on seed bed by using line sowing method (Genc 2012). 50 seeds were sown in each line and sand-forest soil mixture was used as cover material. The irrigation in greenhouse was done by automatically. The temperature of morning, noon and evening in the greenhouse was measured by thermometer and recorded regularly from the beginning of germination to the end of germination. Germinants were recorded weekly. Statistical Analysis – Statistička analiza In the present study, data were analyzed using the SPSS statistical software. Correlation analysis, Analysis of variance (ANOVA), Duncan’s test and Independent samples t-test were used (Ozdamar 2010). RESULTS REZULTATI Laboratory Experiments – Istraživanja u laboratoriju The seeds collected at three different times were germinated in the growing chamber at + 5 0C after they had been subjected to soaking 48 hours water, 8 week stratification and GA3 applications (50, 100 and 400 ppm). The difference between the treatments was tested by analyses of variance and the significance of differences between groups was tested by Duncan’s test (Table 2 and Table 3) |