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ŠUMARSKI LIST 3-4/2020 str. 51     <-- 51 -->        PDF

Laboratory and Greenhouse experiments – Pokus u laboratoriju i u stakleniku
In this study, treatments of growth chamber and greenhouse are shown below (Table 1).
Laboratory Experiments – Pokus u laboratoriju
The seeds were soaked for 48 hr in water at room temperature (Genc 2007) before germination and sowing treatments in order to break dormancy caused by seed coat. Also, the seeds were treated with cold-moist stratification treatment to break seed dormancy. The seeds were mixed with approximately % 40 humidified sand and placed in plastic bags, and then stored in the refrigerator (at 4 0C) for cold-moist stratification treatment (Saatcioglu 1971; Jensen 2001; Yahyaoglu and Olmez 2004). In pre-experiments, the highest germination percentage of stratified seeds was obtained in seeds treated with stratification for 8 weeks. When the stratification period was prolonged, most of the seeds germinated during stratification period. Therefore, stratification priod was determined as 8 weeks in this study. As a different treatment, the seeds collected different collection time were treated with GA3 (Giberellic acid; 50,100,400 ppm) for 24 hr and germinated in growth chamber (Table 1).
Germination tests were conducted in petri dishes covered with filter paper (ISTA 1996) and 100 (4 X 25) seeds were used for each germination test. Petri dishes and filter paper were sterilized in the oven at 160 oC for approximately 2 hours. Also, the seeds were sterilized in a % 2 sodium hypochlorite solition for 10 minute and rinsed in pure water for 5 minute before germination treatments (Jensen 2001). Petri dishes covered and randomly placed in growth chamber. The seeds with radicles longer than 3 mm were tought to be germinated and taken from the petri dishes (Jensen 2001). Germination tests were considered completed when there was no germination for 14 consecutive days (Tremblay et al. 1996). In pre-experiments, the highest germination percentage of Acer cappadocicum seeds was observed in germination experiments at + 5 0C. Therefore, in this study, the germination experiments were carried out at + 5 0C.
Greenhouse Experiments – Pokus u stakleniku
In order to evaluate the germination performance of Acer cappadocicum seeds in the greenhouse conditions, seed beds (soil ) were used in the greenhouse at East Black Sea Forestry Research Institue. The seeds were subjected to soaking 48 hr water, 8 week stratification, soaking 48 hr water + 8 week stratification and GA3 applications ( 100 and 400 ppm) (Table 1).100 seeds were used for each treatment. The seeds were sown on seed bed by using line sowing method (Genc 2007). 50 seeds were sown in each line and sand-forest soil mixture was used as cover material. The irrigation in greenhouse was doneby automatically. The temperature of morning, noon and evening in the greenhouse was recorded regularly from the beginning of germination to the end of germination. Germinants were recorded weekly.
Statistical Analysis – Statistička analiza
In the present study, data were analyzed using the SPSS statistical software. Correlation analysis, Analysis of variance (ANOVA), Duncan’s test and İndependent samples t-test were used (Ozdamar 1999).
RESULTS
REZULTATI
Laboratory Experiments – Pokus u laboratoriju
The seeds collected at tree different times were germinated in the growing chamber at + 5 0C after they had been