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|ŠUMARSKI LIST 11-12/2018 str. 37 <-- 37 --> PDF|
employment of the statistical program SPSS for Windows 20.0 Evaluation Version. Differences between control and treatment groups were analyzed through utilization of one-way ANOVA. After the variance analysis, Tukey multiple test was employed to determine differences in significance value of P <0,05. In addition, the “Correlation Analysis” was performed to determine statistical relations between seed biochemical characteristics and “Spearman Correlation Coefficient” for non-normal distribution characteristics were taken into consideration.
The data related to the chemical composition and antioxidant activities of Taurus cedar seeds from different populations, have been given in table 2 and 3. According to the results of the variance analysis and multiple test results, there are significant differences between the levels of the mentioned compounds for different populations (p<0,05).
Proline, total soluble protein, MDA and H2O2 amounts – Prolin, ukupni topivi protein, MDA i H202
Proline amounts in the sample seeds varied significantly among different populations (p<0.001). The highest proline value weredetected in ANA and POZ populations representing the optimum core area and the marginal-isolated NIK population from the northern distribution area. The lowest proline amount were detected in the KAS, AND and FIN population samples. As for the total soluble protein amounts, the highest values were obtained from the seed samples of FIN and KAS populations, and the lowest values were detected in ANA. High MDA concentrations were detected in NIK and AND population samples and low MDA concentrations were detected in KAS and FIN populations, two close locations. H2O2 concentrations in the seed samples varied between 4,68 and 11,97. The highest H2O2 amounts were detected in the NIK and ANA population samples (Tab. 2).
Antioxydant activities – Antioksidacijske aktivnosti
In the seed samples, APX activities varied between 0,962 Enzyme Unit (EU) and 0,248 EU. The highest APX activity was detected in POZ samples, and the lowest APX activities were detected in AND, KAS and FIN samples. The highest CAT activity was detected in POZ population (0,827 EU), whereas the lowest value was detected in FIN population (0,465 EU). ANA population exhibited the highest GuPX activity; and the seed samples of AND and KAS populations exhibited the lowest activity. The highest SOD activity was detected in the seed samples of FIN, KAS and NIK, in descending order. α-amylase activity in the seeds varied between 29,26 EU and 13,42 EU. The highest α-amylase activity was detected in the seed samples of POZ, NIK and ANA, whereas the lowest values were detected in the samples of KAS and FIN (Tab. 3).
DISCUSSION AND CONCLUSION
RASPRAVA I ZAKLJUČAK
According to the literature, seed characteristics such as the seed morphology, physiology and biochemistry may vary depending on several factors such as genotype, variability of environmental factors, growth physiology of seeds, preharvest and postharvest processes and the interaction between these factors (McDonald, 2004).In addition to their role in the growth and development of embryo, proteins are also effective in increasing the resistance against abiotic and biotic stress factors that seedling undergoes during germination (Halliwell, 2006; Bewley et al., 2013).
In this research, the highest total soluble protein amount was detected in FIN and KAS populations, and the lowest amount was detected in ANA population (Table 2). Detection of the highest protein amount in FIN and KAS populations is attributed to low MDA concentration and high SOD activity (Foyer and Noctor, 2005; Caverzan et al., 2012). On the other hand, low protein amount in ANA samples is ascribed to high H2O2 concentration (Bailly, 2004; Halliwell, 2006). Also, detection of the highest proline