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ŠUMARSKI LIST 7-8/2006 str. 14     <-- 14 -->        PDF

D. Ballian: KONTROLA PODRIJETLA PANČIĆEVE OMORIKE (Picea omorika /Panč./ Purk.) ... Šumarski list br. 7–8, CXXX (2006), 295-304
SUMMARY: In this study we tried to identify and determine the origin of
the Serbian spruce from the Kakanj plantation, covering the space of 8,2 ha,
with the means of molecular genetic analysis. We applied the isoenzyme
markers analysis to verify the obtained data from the molecular genetic identification
of natural populations of Serbian spruce, and those from the said
plantation. A total of 12 enzyme systems with 16 gene loci have been used,
with 24 alleles analyzed.

Based on the statistical analysis in the computer program BIOSYS-1
(Swofford & Selander 1981), according to Ballian et. al (2006), the genetic
variability of 12 natural populations, where 16 isoenzyme loci were studied,
strong differentiation was obtained (Fst = 0,261), but with exclusion of the
Kakanj plantation which exhibited a rather low differentiation. Here we cannot
speak about the presence of geographic genetic differentiation in the cline
form. It was determined that alleles with the studied populations had randomly
distributed frequencies. Out of the obtained results it is apparent that
Serbian spruce plantation in Kakanj is poorly differentiated, and this supports
the opinion of its mixed origin (according to Ballian et. al. 2006). If we
take a more detailed look at the obtained dendrogram, which was obtained on
the basis of genetic distance between the studied populations, where the
Kakanj plantation occupies a totally unexpected position, this can additionally
confirm its mixed origin.

The results obtained through this study, by isoenzyme markers analysis,
show that our present possibilities to confirm the origin of Serbian spruce are
relatively limited. The offered system of identification by this method might in
the future prove acceptable as the initial studies exhibited good results, even
though we still cannot entirely systemize the analyzed plantation to certain
population or populations. We should therefore continue with basic studies
with the aim to create a more detailed genetic picture of the spruce populations,
with larger number of enzyme systems, that is, gene loci, which would
encompass all natural populations, and groups of trees in Bosnia and
Herzegovina and Serbia, which is a long lasting and expensive effort.