DIGITALNA ARHIVA ŠUMARSKOG LISTA
prilagođeno pretraživanje po punom tekstu
| ŠUMARSKI LIST 7-8/2003 str. 27 <-- 27 --> PDF |
D. Ballian: PROCJENA GENETIČKE VARIJABILNOSTI OBIČNE JELE (Abies alba Mili.) ... Šumarski list br. 7-8, CXXVII (2003). 347-357 gen, 1999: High level of variation at Abies alba Ziegenhagen,B.,A. Kormut´ak,M. Schauer- chloroplst microsatellite loci in Europe, Mole- te, F. Scholz, 1995: Restriction site polymor- cular - Ecology, 8 (7): 1117-1126. phism in chloroplast DNA of silver fir {Abies Vendramin, G. G., M. Anzidei, A. Madag- alba Mill), Forest Genetics, 2 (2): 99-107. hiele, C. Sperisen, G. Bucci, 2000: Chlo- Ziegenhagen, B., M. Schauerte, A. Kor- roplast microsatellite analysis reveals the pre- mut´ak,F. Scholz, 1996: Plastid DNA poly- sence of population subdivision in Norway spru- morphism of megagametophytes and pollen in ce (Picea abies Karst.), Genome, 43: 68-78. two Abies species, Silvae Genetica, 45 (5-6): Vukelić, J., D. Baričević, 2001: Šumske zajed- 355-358. nice obične jele u Hrvatskoj, ed. Obična jela u Hrvatskoj, Zagreb, 162-186. SUMMARY: This research was aimed at establishing the molecular-genetic variability of some populations of silver fir in Croatia and Bosnia & Herzegovina (Vranića, Meka Brda, Gorski Kotar - adult, Gorski Kotar — juvenile, Crni Vrh, Cabulja, Orjen). Variability was shown using the analysis ofcp DNA, or more accurately, the number ofhaplotypes (H), effective number ofhaplotypes (Ne), and haplotype differential (Hexp & Sw.). The ep DNA analysis was used to study the haplotype variability of the silver fir. The obtained results show considerable differences among populations. With the use of chloroplast DNA, the number ofhaplotypes (H), the effective number of haplotypes (Ne), and the haplotype differential (Hexp & Sw), differences were found between the studied populations, and particularly between small, isolated and large populations. No major differences were established between adult and juvenile populations of Gorski Kotar (A & A). Since their genetic variability is insignificant, there are practically no differences in these populations. However, the results point to the need for a more detailed study at the molecular level. In order to set up and maintain genetic resources, a dense network of gene databases should be established both in situ and ex situ necessary for the preservation of genetic differences among populations. Accordingly, every ecological niche important for the silver fir should have its own genetic database with a corresponding number of units so that the eco-physiological characteristics of each population could be preserved. In managing natural resources and their renewal, natural regeneration should always be favoured, combined with constant monitoring of the genetic structure, so that proper measures could be undertaken to ensure the genetic variability that characterises every population. Future research should be directed at other populations of silver fir in Croatia and Bosnia and Herzegovina. An optimal number of individuals should be established in a sample. Management and regeneration of the silver fir should constantly be monitored and directed towards the maintenance of genetic differences within local populations. Moreover, numerous experiments should be conducted with different provenances in order to investigate eco-physiological properties of the silver fir. 357 |