DIGITALNA ARHIVA ŠUMARSKOG LISTA
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J. Rosa: UTJECAJ FOSFOGIPSA IZ INDUSTRIJSKOG ODLAGALIŠTA NA HRAST LUŽNJAK (Querem rohur) Šumarski list br. 11-12, CXXII (1998), 491-506 in Picea abies ( L). Karst, and its possible role in lyses in determination of damage degree of com the "Fichtensterben". European Journal of Forest mon oak forests. Radovi 29( 1), 151 -160. Pathology 16,408-423. Zoldoš, V., Besendorfer, V., Littvay, T., PaSchlegel, R., Craig, R.N. 1991: Mitosis: Normal pes, D. 1994b: The common oak (Quercus rocontrol mechanisms and consequences of aber-bur L.) as potential test plant for cytotoxicyti rant regulation in mammalian cells. U: Perspe- monitoring. Periodicum Biologorum 96 (4), ctives on cellular regulation: From bacteria to 490-492. cancer (J. Campsi, D.D. Cunmngam, M. Riley Zoldoš, V., Besendorfer, V ., Jelenić, S., Loreds), wiley-liss, inc., New York, 253-249. ko v i Ć, Z., Littvay, T., Pape š, D. 1997: Cy- Thijsse G.,Baas,P. 1990: "Natural" and NH3 indu- togenetic damages as an indicator of pedunculate ced variation in epicuticular needle wax mor- oak forest decline. U: Borzan, Ž. Schlarbaum, phology of Pseudotsuga menziessi. Trees: Struc- S.E. (ed.), Cytogenetic Studies of Forest Trees ture and Function 4, 111-119. and Skrub Species. Proc. First IUFRO Cytoge- Zoldoš, V, Li ttvay,T, Besendorfer, V., Jele - netics Working Party S2.04-08 Symposium, Brinić, S.,Lorković,Z.,Papeš,D. 1994a: The juni 8-11, 09.1993.275-284. application of citogenetic and biochemical ana- SUMMARY: Decline and decay of pedunculate oak forests (Quercus robur L.) becomes a more and more important problem of forestry in Croatia. Decay of forests is obvious near industrial formations and their dumps. The effect of phosphogypsum and waste waters of fertiliser factories in Kutina on pedunculate oak forests which are located near these factories is examined in this elaboration. The chemical analysis of the water from the dumps has proved the presence of heavy metals (AI, Cd and others), as well as a remarkable low pH value of 1,2-2,6. The pedunculate oak growing in the surrounding of the dump showed 60-70% of the crown damage and therefore belongs to the category of highly damaged trees. As a first step in our investigation we applied a short-term cytotoxicity-test. Root-tip meristematic cells were treated by different dilution rates (from 0,5% to 10%>) of phosphogypsum from the dump. Roots were excised from the sprouts germinated in laboratory from the acorns gathered in the tree seed stand. Mitotic activity decreased with an increase of concentration, except for the concentration of 2,5% where the opposite effect was noticed. All concentrations caused the same cytotoxic effect e. i. mitotic aberrations such as c-mitosis and lagging chromosomes appeared as a result of the spindle disturbances. The most frequent were subchromatide aberrations such as sticking of the chromosomes. There was no significant change of the mitotic activity after a 24-hour recovery for any of the concentrations. Mitotic activity remained low and even higher rate of the subchromatide aberrations were noticed. Allium-test was applied as a control test of the cyto- and genotoxicity of the phosphogypsum from the dumps. Bulbs of the onion Allium ascalonicum were germinated in the tap water and treated subsequently with the same dilutions of phosphogypsum that were used in cytotoxicity test with roots of pedunculate oak. As a negative control we used onions germinated in tap water while onions treated with maleichydrazide served as a positive control. Allium-test confirmed cyto- and genotoxicity of phospogypsum from the dumps. Important inhibition of the roots´ growth after a five-days-treatment |