DIGITALNA ARHIVA ŠUMARSKOG LISTA
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ŠUMARSKI LIST 1-2/1991 str. 33 <-- 33 --> PDF |
11. Karnosky , D. F. (1981): Potential for forest tree improvement via tissue culture. BioScience 31 (2): 114—120. 12. Kolevska-Pletikapić, B. (1985): Klonsko razmnožavanje Leuce topola metodom kulture tkiva. Topola 145—146: 3—8. 13. Kolevska-Pletikapić, B., Z. Tomović (1988): Mikropropagacija bagrema. Šumarstvo 5—6: 29—35. 14. Pe valek-Kozlina, B., S. Jelaska (1986): In vitro growth and development of oaks (Quercus robur and Q. petrea). Acta Botanica Croatica 45:55—61. 15. Sellmer, J. C, B. H. McCown, B. E. Haissig (1989): Shoot culture dynamics of six Populus clones. Tree Physiology 5:219—227. 16. Tu co vić, A., M. Jovanovi e (1970): Međuvrsna hibridizacija breza sa istim i različitim brojem kromosoma. Zbornik Instituta za šumarstvo i drvnu industriju 9:215—226. 17. Welander , M. (1988): Biochemical and anatomical studies of birch (Betulapendula Roth.) buds exposed to different climatic conditions in relation to growth in vitro. U: W. Hanover, D. E. Keathley (Eds.) Genetic Manipulation of Woody Plants. Basic Life Sciences, Vol. 44. Plenum Press. New York, pp. 243—264. Micropropagation of European birch (Betula pendula Roth.), paper birch (B. papyrifera Marsh.) and yellow birch (B. lutea Michx.) S u m m a r y The vegetative propagation by conventional methods which exploit natural mechanisms of asexual reproduction, rooting of cuttings and grafting, is time consuming and of limited success for many tree species. Tissue culture techniques offer a new dimension to tree improvement and may serve as an adjunct to conventional tree-breeding programs. In present paper we described how micropropagation technique could bee used in production and genotypes selection of three birch species Betula pendula, ß. papyrifera and B. lutea. Initial explants — axillary bud meristerns, were excised from two-, four- and twenty-year-old European birch, as well as from two-year-old paper and yellow birch. The branches were collected in winter to early spring before foliation. The modified aspen culture medium (ACM) with low concentration of cytokinin BA (2.2 u.M) was used as initial medium for shoot induction and multiplication. The differences observed in all micropropagation stages were affected by different morphogenetical capacity of tested species as well as their genotypes. Multiplication rate achieved was 4—6 nodal segments per explant. The adventitious root system was developed on the basal portion of shoots on the same medium composition (mod. ACM) supplemented with 1.0 u.M IBA. Plantlets which passed through hardening period, were developed into the normal plants in field conditions. The results presented in this paper showed, also, that our micropropagation method could bee used for propagation of mature birch trees. |