DIGITALNA ARHIVA ŠUMARSKOG LISTA
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ŠUMARSKI LIST 1-2/1968 str. 70     <-- 70 -->        PDF

Prilikom klijanja polena molike na ravnom staklu u Petrijevim šoljama na
rastvoru glikoze + 2% agara, dobiveni su vrlo slabi rezultati.


2. Klijanje polena Pinus peuce na sobnoj temperaturi gde se ona kolebala
između 15 (noću) i 25° (danju) daje vrlo dobre rezultate. U toku prva dva dana
klija mali deo polenovih zrnaca (oko 25% od ukupne količine), ali do kraja ogleda
(svaki je ogled trajao po 6 dana) veći deo polena je proklijao.
3. Najveći procenat proklijalih polenovih zrnaca zapažen je, i to kod sva
tri ogleda, između drugog i trećeg dana.
LITERATURA


1.
AljbenskiA . V.: Selekcija drevesnyh porod i semenovodstvo. Moskva, 1959.
2.
Bern er H. - C h r i s t i a n s e r: On the extraxtion of Forest Tree Pollen frominflorescens.
»Silv.ae genetika«. No. 7. 1958.
3.
Worsley : The processing of Pollen. »Silviae genetika«. 1959.
4.
Worsley : Pollen fractionation — a method of increasing the viability of
Pollen samples. »Silvae genetika«. 1959.
5.
Duff i eld: Studies of extraxtion storage and Testing of Pine. »Zeitschrift für
Forsgenetic« No. 3. 1954.
6.
Dakov-Dobrinov : Genetika i selekcija na drevesnite vidove (II čast). Sofija,
1962.
7.
Jablokov : Selekcija drevesnyh porod. Moskva, 1962.
8.
Kantor J.-Pospišil J.: Pešteni lesu (Ideo). Praha, 1960.
9.
Kotelova-Ljubavskaja: Rukovodstvo k vypolneniju laboratornyh zanjatij
po selekciji. Moskva, 1962.
10.
Lehotsk y L.: Šlachtenie lesnych drevin. Bratislava, 1965.
11.
Mamae v S. A.: Biologičeskie osobennosti pyljey sosny iz razlicnyh rajonov
Urala. »Introdukcija i selekcija rastenij na Urale«. Vypusk 42. Sverdlosk, 1965.
12.
Nekrasov a T. I.: O značenii žoltoj i rozovoj okraski mužskih šišek u vidov
Pinus. »Bot. žurn.« br. 7. Moskva, 1959.
13.
Pjatnicki : Praktikum po lesnoj selekciji. Moskva, 1961.
14.
Pospiši l J.: Cvičeni že šlechteni lesnych drevin. Praha, 1959.
15.
Stanle y R.: Viable Pine Pollen Stared 15 Years Produces Unsoud Seed »Silvae
genetica« No. 5—6. 1962.
16.
Chir a E.: O nakličovani pelu druhu Pinus edulis Engelnanii s viae ako haploidnim
poekom chromozomov. »Biologija« No. 4. Bratislava, 1967.
BIOLOGY OF THE GERMINATION OF PINUS PEUCE GRIS. POLLEN
UNDER LABORATORY CONDITIONS (»IN VITRO«)


Summary


When testing the germination of Pinus peuce pollen »in vitro« under varying
room temperatures the author has established as follows:


1. Freshly collected pollen of Pinus peuce germinates best on 3"/o-, 25´Vo-, 20B/oand
1´%-glucose solution + 2°/i>-agar solution.
Nearly the same results (apart from insignificant differences) were obtained by
the author when using glucose solution alone. In this case the best results were obtained
when the following concentrations of glucose were used: 25;l/o, 10D/o, 3% and
5´%.


When letting the pollen of Macedonian Pine (Pinus peuce) germinate on flat
glass in Petri-dishes on glucose + 2n/o-agar solutions very poor results were obtained,
for which reason this method is not recommended.


2. The germination of Pinus peuce at room temperatures fluctuating between
15´ (at night) and 25° (in daytime) was very good. During the first two days germinates
a relatively small percent of pollen grains (ca. 25´Vo of the total amount) but
by the end of the sixth day (which was the duration of each experiment) the majority
of pollen grains germinated.
3. The highest percent of germinating pollen grains was noticed to occur in
most cases between the second and third days of pollen sowing.
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